What type of media should I use to grow a lemontyme bonsai tree? Please help!?
I am a beginner bonsai artist. I chose lemon tyme because it is cheap and it seems easy to grow. This is going to be my first bonsai tree. I am not sure of what potting mix or growing media I should use for this, and what type of container to put it in. I am asuming it would go good in a shallow pot.
Suggestion by ❤Eclair
My mom grew a bonsai tree one time, she had it for 40 years. She went to a professional plant store called Sunny Panzico’s, and they told her that she could use regular potting soil for her bonsai tree, and it was a success.
Suggestion by Jack
it all depend on the size bonsai you want…if you want it in a shallow pot (some people like decently deep pots or very shallow trays either way this will help)you just cut the roots that grow strait down and then leave the ones that grow out and then just use normal potting mix id say is fine just don’t over water cause the tree will get water logged and die so if you want its a good idea to get soil that drains well…and when you pot the plant poke some wire from the top of the roots and through the drain hole (its best to get one with 2 drain holes)in the bottom to secure the plant basically you want the wire to look like this ____/ only the upside down so when you poke the wire though the holes you can bend the wire at a 90 degree angle so you can keep the tree from falling over before it takes root….and when you decide you want to wire the branches use a wire that is only half the size of the branch your bending and always use aluminum or brass…then let it grow and prune the tree to desired shape try not to do this all in one year cause it can kill the tree.
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How can bacteria auxotrophic for arginine grow on medium lacking arginine?
You have a strain of bacteria that normally grows in the absence of the amino acid arginine. You plate a large number of these cells on rich medium and then replica plate to medium lacking arginine. You isolate cells that are now auxotrophic for arginine and grow a large culture of these cells to plate on medium lacking arginine and 6 bacteria colonies grow. Explain 2 different ways that these 6 colonies could have grown on medium without arginine.
Suggestion by Nick
One way would be that you got revertants of the original mutants unable to make arginine. Whenever you grow large batches of bacteria you’re going to have a certain amount of mutation. In your original selection you chose mutants that likely had a mutation in some gene essential for arginine biosynthesis. When you grow up a large bunch in a flask it’s possible to have a handful that will either mutate back to the original sequence or will have a second mutation that compensates for it.
Another way auxotrophs could grow would be if they could obtain enough arginine from dead cells around them. When you grow a bunch of cells in regular media they will incorporate arginine into their proteins as well as build up stores in the cytoplasm. When they are plated on -arg media and starve to death, this arginine will be released, and a lucky few bacteria might be in an opportune spot to collect this released arginine.
Suggestion by VandyVan
That’s due tomorrow morning at 9:10 A.M. in room 1220 MRB III. I would get hopping on that one.
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